Mitochondrial dna 4977 bp deletion among sudanese oral lesions

Background/Objectives: Recently, oral cancer mortality and morbidity is very high in Sudan, particularly among men due to environmental factors and habits of life style. The study aimed to investigate the frequency of mitochondrial DNA (Dloop) mutations (mtDNA4977) in oral lesions. Materials and Methods: This is a retrospective study on oral lesions biopsies in Khartoum state Sudan. The data were collected from examination of biopsies and patients records. All oral biopsies were examined and classified into histopathological pattern by using hematoxylin & eosin standard method. The detection of mtDNA4977 bp deletion was performed using a PCR-based technique. Data was analyzed by using the statistical package for social sciences (SPSS) for Windows computing program version 20. Results: One hundred and fifteen oral biopsies were taken from patients during surgical operation (61 male; 54 female), their mean age was 47±18. Of the study samples, 81 (70.4%) samples were malignant, 18 (15.7%) were benign and 12 (10.4%) were inflamed. Squamous cell carcinoma was the most frequent 55 (67.9%) of malignant cases, followed by adenocarcinoma 15 (18.5%), mucoepidermoid carcinoma 5 (6.2%), ameblastoma 4 (4.9%), and lymphoma 2 (2.5%). Four of the malignant cases (1 male and 3 female) were having the 4,977 -bp deletion. ejpmr, 2015,2(4), 32-43 SJIF Impact Factor 2.026