Investigating IκB Kinase Inhibition in Breast Cancer Cells Biomarker Discovery on the TripleTOF ® 5600 System Using iTRAQ ® Reagent Labeling

The IκB kinase (IKK) pathway is important in tumourigenesis and is known to be mis-regulated in a number of cancers including breast cancers. IKK is primarily known for its central role in the activation of NF-κB, which may contribute to tumourigenesis through regulation of the cell cycle and evasion of apoptotic signals. In this research study, the effect of a specific IKK inhibitor, IMD0354, on the T47D breast cancer cell line was investigated. T47D cells are an ER+ breast cancer cell line with an intact IKK signaling pathway (Figure 1).