A novel platform for engineering the tumor 3 d microenvironment

ECM was isolated from human calf muscle and placenta by decellularization followed by lyophilization. Lyophilized ECM was milled to a fine powder and subjected to enzymatic digestion. LatBioGel was generated by combining ADSC and tissue ECMs according to a proprietary process To evaluate DNA content, total DNA was isolated from 25 mg of ECM and measured by absorption at 260 nm. Total protein amount in ECM was evaluated using the BCA protein assay followed by absorbance measurement at 562 nm. western blot analysis was conducted on loads 25 μg of ECM.