Cemmunication Elucidation of the Role of Sugar Chains in Glycosylated-enzymes Using Endo-fi-IVLacetylglu-cosaminidase from IJIIavo-bacterium sp

isolated. from a soil sample produces a very high amount of endo-fi-IVLacetylglucosaminidase (endo-6-GlcNAcase) in the culture fluid. We identified the bacterium as rvavobacterium sp., and reported the purification and properties of the endo-fi-GlcNAcase.i) Here we report that the carbohydrate moieties of glycoenzymes may protect them against proteolysis. The term glycoenzyme has been used fbr enzymes that contain covalently linked carbohydrate residues in their structures,2} Yeast invertase, a representative glycoenzyme, contains a large amount of carbohydrates, about 50% of its molecular weight of