Oral Response to a Newly Formulated Autopolymerized Complete Denture Base Material ( Microbiologial and Cytological Study )

Statement of problem: Several alternative methods to conventional compression-molding processing for denture base material have been developed to increase the adaptation of the denture base, such as fluid resin techniques. These offered improved adaptation, dimensional stability, reduced cost and simple procedure but had low strength, higher solubility and high residual monomer levels which is related to mucosal irritation and sensitization of tissues. Subjects and Methods: Fourteen Completely Edentulous patients were selected. Patients were divided into two groups equally.one group were treated using auto-polymerized pour-type acrylic resin denture base material and the second group were treated using the conventional heat cured acrylic resin (PMMA) complete denture base material. Microbiological and cytological samples were collected. Data were statistically analyzed.Results:When comparing the two studied groups regarding mean difference of the level of both pro inflammatory salivary cytokines (TNF-α & IL-6) after delivery of the new denture, group I levels were significantly higher than group II levels at each period separately (1⁄2 hour, 1 hour 2 hours, 1 day, 3 days,1 week and 2 weeks)and when comparing between the two studied groups regarding the mean values of the number of keratinized epithelial cells before and after two months of denture delivery, it was found that there was no significant difference between them. Conclusion: Immunologically, Auto-polymerized pour-type acrylic resin denture base material elicited higher immunological response at the beginning due to its chemical composition and leaching of higher amount of residual monomer. Cytologically, both denture base materials showed an increase in the number of keratinized epithelial cells with no significant difference between them.