DEvELOPMENt OF A MEthOD OF stANDARDIzAtION FOR LOw-MOLECULAR PECtINs

DEvELOPMENt OF A MEthOD OF stANDARDIzAtION FOR LOw-MOLECULAR PECtINs E.V. Khozhaenko1, 2, R.Yu. Khotimchenko1, 2, V.V. Kovalev1, E.A. Podkorytova1, M.Yu. Khotimchenko1, 2 1 Institute of Marine Biology named after A.V. Zhirmunskiy Far Eastern Branch of RAS (17 Palchevskogo St. Vladivostok 690041 Russian Federation), 2 School of Biomedicine of Far Eastern Federal University (8 Sukhanova St. Vladivostok 690950 Russian Federation) background. Object of this study was developing a method of stan‐ dardization of low‐molecular pectins‐oligouronids according to the chain‐length distribution for subsequent validation. Methods. Standardization of oligouronids was carried out using ex‐ clusion chromatography techniques and analysis of reducing end groups. The authors studied the eluent influence on the separation of oligouronids with molecular weights of 2.8–15.5 kDa on column with hydrophilic sorbent Shodex Asahipak GS‐320 7E. Results. It has been established that the optimal separation of oli‐ gouronids on molecular weight is achieved in 0.1M of sodium ni‐ trate at 35 °C and the eluent speed of 0.8 ml/min. The values of mass average and number average for oligouronids molecular weights obtained respectively with the exclusion chromatography methods and analysis of the end groups were similar that indicates a rela‐ tively small value of polydisperse samples and the validity of these methods for standardizing oligouronids. Conclusions. When comparing the modified tetrazolium method and Nelson method for analyzing oligouronids end groups it was determined that Nelson method allows to obtain more accurate and reliable results and it is applicable for oligouronids standard‐ ization.