Biosynthesis of 2O-α-D-glucopyranosyl-L-ascorbic acid from Maltodextrin Catalyzed by Cyclodextrin Glucanotransferase from Bacillus sp . SK 13 . 002

Cyclodextrin glucanotransferase from Bacillus sp. SK13.002 has specificity to maltodextrin as a glycosyl donor for production of 2-O-α-D-glucopyranosyl-L-ascorbic acid. In the present investigation, the product was analyzed by HPLC and the reaction parameters were optimized. The results showed that the optimum reaction condition was pH 8 at 37C for 24 h with 1:1 maltodextrin to L-ascorbic acid ratio and 80 U/mL of Cyclodextrin glucanotransferase. Under the above optimal conditions, the production of AA-2G was 6.3 g/L, indicates that Cyclodextrin glucanotransferase from Bacillus sp. SK13.002 is an effective producer of AA-2G compared to previous studies.