Teniposide Leukemic Lines : Effects of Methotrexate , Camptothecin , and Normal Human Lymphocytes and in Cells of HL-60 and MOLT-4 Cell Cycle-related Expression of p 120 Nucleolar Antigen in Updated

Expression of the proliferation-associated nucleolar antigen pi 20 was studied by flow cytometry in human quiescent and phytohemagglutininstimulated lymphocytes, as well as in human lymphocytic (MOLT-4) and promyelocytic (HL-60) cell lines. Divariate analysis of pl20 and DNA content made it possible to correlate p120 expression with cell position in the cycle. Proliferating lymphocytes and MOLT-4 and HL60 cells had a similar pattern of pi 20 expression. Populations of Gt cells, in all three cell types, were very heterogenous with respect to p120, and a threshold in (., was observed. The cells with a pi20 level below the threshold value did not enter S phase. An increase in pi 20 was observed during progression through S phase, and the antigen was maximally expressed in (.. cells. The pi 20/1)\.A content ratio, however, was highest in late Ci cells (更多