Guanidine containing phosphate buffer system ― ―

1)ペンタックス株式会社 ニューセラミックス事業部 〒174―8639 東京都板橋区前野町2―36―9 TEL : 03–3960−1290FAX : 03–3960–2681 E−mail : tomo.yoshitake@aoc.pentax.co.jp 2)プロテインテクノスインスティテュート 〒243―0206 神奈川県厚木市下川入99―7 Abstract Elution behaviors of guanidine denatured proteins on ceramic hydr ox apatite column with 2 M guanidine c ontaining phosphate buffer system were investigated. In phosphate buffer system, the retention time of pr oteins was increased with the positive charge of the proteins. On the other hand, in guanidine containing phosphate bu ffer system, the retention time of the proteins decreased with the positive charge of the proteins. Guanidine denatured acidic and neutral proteins were retained o n the column, however the most of the basic proteins were not retained on the column, except cytochrome−c. In sulfonate type cation−exchange chromatography as comparison, showed no retention of cytochrome −c with 2 M guanidine buffer system.