Uv spectrophotometric method development and validation of lopinavir in bulk and in pharmaceutical dosage form *

A new, simple, precise and accurate UVspectrophotometric method was developed and validated for the determination of lopinavir in pure and tablet dosage form. Lopinavir exhibited maximum absorption at 203nm in methanol and obeyed linearity in the concentration range of 10-50 μg/ml. The proposed method was statistically validated. From the results obtained for precision, it was found that % RSD is less than 2% it indicates that the proposed method has good reproducibility. The linearity coefficient of lopinavir was found to be 0.999 and accuracy it was found that percentage recovery values of pure drug from the analyzed formulation was between 98.6-102.2 which indicates that the method is accurate and commonly used excipients and additives present in the formulation was not interfering in the proposed method. The UV-spectrophotometric method was extensively validated for linearity, accuracy, precision, LOD and LOQ. All these analytical validation parameters were observed and the % RSD was determined which indicates the usefulness of method for determination of lopinavir in bulk drug and tablet formulation.