Purification and Characterization of a var . Mexico Seeds Lectinfrom Amaranthus ilypochondviacus

Munetaka, OzEKi, Kazuo KAMEMuRA, Kikuko MoRiyAMA, Yayoi,Hayato UMEKAwA,Takao TAKAHAsHi,Mie Uhiversity, Yukio FuRulcHI

semanticscholar（2018）

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Abstract

A 1ectin from Amaranthus iij{pochendeiacns var. Mexico (AHML) was purified by aMnity chromatography using asialofetuin-Sepharose 4B, AHML is specific for N-acety1-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pl of AHML is 6.8. AHML has a natiye molecular mass of 45.0kDa and is composed of homo-subunits having molecular masses of 36.8kDa.

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