Effect of crude oil contaminated soil on phaseolus aureus and its bioaugmentation by pseudomonas aeruginosa ch23

Petroleum hydrocarbons are the most obvious pollutants in both terrestrial and aquatic realm. Its seepage in agriculture field can create toxic conditions and harm valuable crops. In the present study, effect of Bioaugmentation/ Bioremediation of such crude oil contaminated soil on mung beans is studied by using an indigenous isolate identified as Psedomonas aeruginosa CH23. A pot study was carried out to study effect of augmented (amended) crude oil contaminated soil on mung beans (Phaseolous aureus) along with controls such as un-augmented contaminated soil and normal soil. The artificially crude oil contaminated soil (5%) was amended with test organism (appx. 5.2x10CFU/gm of soil). Various parameters like number of seeds germinated, height, wet weight of plants and chlorophyll a and b content of the leaves were studied after every 5 days till 30 days. The pot study confirmed that the organism Pseudomonas aeruginosa CH 23 is not only effective in degradation of crude oil in laboratory shake flask condition but could also compete with indigenous soil microorganisms in in-situ bio-degradation as well in decreasing the phytotoxicity of crude oil.