PREPARATION AND SPECTROSCOPIC CHARACTERIZATION OF INCLUSION COMPLEX OF 2- PHENYL-4H-BENZO[d][1,3]OXAZIN-4-ONE AND β-CYCLODEXTRIN

Objective: Quinazoline-4-(3H)-one derived drugs are emerging as new generation drugs, which motivated us to study some special features of the molecules. The major predicament is their low water solubility, which often gives unreliable results in biological experiments. In the present study, we have tried to solubilize the molecule making a host-guest complex with β-cyclodextrins with the expectation that it may develop new formulation of the stated molecule. An optimized method for the complexion of 2-phenyl-4H-benzo[d][1,3]oxazin-4-one with β – cyclodextrin is reported herein. Methods: 2-phenyl-4H-benzo[d][1,3]oxazin-4-one was synthesized following standard method. Different methods were tried to encapsulate the molecule into the cavity of cyclodextrin. The compound and its complexation with βcyclodextrin were analyzed by spectral methods. Results: The result showed that the encapsulated 2-phenyl-4H-benzo[d][1,3]oxazin-4-one into the cavity of β-CD fitted better and thus improved its solubility. It was also observed that kneaded method is the best way to prepare the host guest complex. Conclusion: 2-phenyl-4H-benzo[d][1,3]oxazin-4-one fits better in βcyclodextrin and an optimized method for the preparation of 1:1 complex is derived.