Svf cell-seeded tevgs and the removal of the in vitro dynamic culture period

INTRODUCTION Tissue engineered vascular grafts (TEVGs) seeded with autologous cells offer a treatment alternative to current coronary artery by-pass procedures1-4. Past work has demonstrated that TEVGs seeded with only a mesenchymal stem cell (MSC) population will remodel in vivo5. However, the isolation procedure of MSCs is approximately a 4-week process6; too long to be feasible in a surgical environment.