Biosynthesis of Albumin via a Precursor Protein in Morris Hepatoma 5123 tc 1 ' 2

The mechanism of albumin biosynthesis was studied in Morris hepatoma 5123tc in vivo and in hepatoma cell sus pensions obtained by solubilizing the intercellular matrix with collagenase and hyaluronidase. In the in vivo experiments, L-[1-'4C]leucine was injected i.v. into rats bearing hepatomas in the muscles of both hind legs. After 14 mm, tumors were removed and homogenized. A protein fraction quantitatively precipitable with antialbu