A simple , fast , inexpensive and efficient method for leukocytes separation with preservation of morphology and cell viability for use in education and research

E.A. Kavati, T.M. Hosada, V. Szulczewski, E. Leão, P. Borelli and A.M. Cianciarullo Laboratory of Genetics, Butantan Institute, Av. Vital Brasil 1500, 05503-900 Sao Paulo, SP, Brazil. Program of Post Graduate Interunits in Biotechnology USP-IBU-IPT, Biomedical Sciences Institute, University of Sao Paulo, Av. Prof. Lineu Prestes 2415, 05508-900 Sao Paulo, SP, Brazil. Program of Professional Improvement, Butantan Institute, Secretary of State for Health, Av Dr Enéas de Carvalho Aguiar 188, Sao Paulo 05403-000 SP, Brazil. ELSME Dr. Elizabeth Leão Medical Services Specialized S/C Ltda, São Joaquim Hospital, Real and Meritorious Portuguese Charitable Association, R. Martiniano de Carvalho 694, São Paulo 01321-000 SP, Brazil. School of Pharmaceutical Sciences, Department of Clinical and Toxicological Analyses, University of Sao Paulo, USP, Av Prof Lineu Prestes 580 Ed. B17, Butanta, Sao Paulo 05508-900 SP, Brazil.