A molecular approach for the detection of Hepatitis B virus Genotyping it ' s clinical importance by using PCR in a Teaching Hospital

Objectives and Aim We performed a study on Seroprevalance, risk factors and HBV genotypes among the patients attending a tertiary care hospital in India. Background: Globally, Hepatitis B is one of the most common infectious diseases. It is ranked by the WHO as one of the top ten killers. The virus is responsible for approximately 1.5 million deaths worldwide in each year, two thirds of which are attributable to primary Hepatocellular carcinoma following HBV infection. About 360 million people are estimated to be chronically infected with HBV. Method -284sampleswerepositiveforHBsAgbyELISA.PCRamplificationwasdonefordetectionof„S‟geneandthesesamplesw eresubjectedtofurtherGenotyping Results-TheseroprevalenceofHepatitisBinfectioninourstudywas6%, associated with blood transfusion as the major risk factor. ThemostpredominantgenotypewasC-66(50.8%) ----------------------------------------------------------------------------------------------------------Date of Submission: 12-10-2018 Date of acceptance: 27-10-2018 -------------------------------------------------------------------------------------------------------------------------------------