Tumor cell pH detection based on 1 fluorescence characteristics of CdSe quantum 2 dots 3

The incidence of cancers has increased year by year and the early diagnosis for cancer is important to prevent cancers. 7 Due to abnormal metabolism of tumor cells, the acidification of extracellular environment is an important feature for cancer 8 cells. To achieve efficient and accurate pH measurement, this paper proposes a new detection method based on the fluorescence 9 characteristics of CdSe quantum dots detection, which uses CdSe QDs with unique optical properties as sensitive substances 10 for pH detection. PBS buffer (0.2 mol/L) with different pH has been used to simulate cell metabolites at different pH values 11 and use 365 nm laser source to excite mixture liquid. The emitted light was detected by a fluorescence detection device. A 12 mathematical model between fluorescence intensity and pH was finally obtained. The experimental results show the method 13 show better effectiveness than other pH detection methods and has a high resolution of 0.037 within a pH range of 6.1–7.8 and 14 a measurement resolution of 0.037 pH units. This method has high temperature stability and short testing time, which shows 15 high potential for pH detection. This method has its superiority in all the testing methods. It exhibits a new way of generalizing 16 to pH detection. 17