Association of SELP genetic polymorphisms and additional gene-smoking interaction on cardiovascular disease in Chinese Han population

Aims: To investigate the impact of P-selectin (SELP) genetic polymorphisms and its interaction with smoking on cardiovascular disease (CVD) risk based on Chinese population. Methods: A total of 1082 subjects (519 males, 563 females), with a mean age of 53.9±13.1 years old, were selected, including 540 CVD patients and 542 normal control participants. Logistic regression model was used to examine the association between four SNP and CVD risk. Odds ratio (OR) and 95% confident interval (95% CI) were calculated. Generalized multifactor dimensionality reduction (GMDR) was employed to analyze the gene-smoking interaction. Results: Logistic regression analysis showed that CVD risk was significantly lower in carriers of A allele of the rs1800805 polymorphism than those with GG genotype (GA+AA versus GG), adjusted OR (95% CI)=1.69 (1.31-2.16). In addition, we also found CVD risk was also higher in carriers of AC genotype of the rs6136 polymorphism than those with AA genotype (AC+AA versus CC), adjusted OR (95% CI)=1.78 (1.28-2.26). GMDR analysis suggested a significant gene-environment interaction between rs1800805 and smoking. Overall, the two-locus models had a cross-validation consistency of 10 of 10, and had the testing accuracy of 62.17%, and smokers with GA or AA of rs1800805 genotype have the highest CVD risk, compared to never smokers with GG genotype, OR (95% CI) was 2.42 (1.83-3.16). Conclusions: Our results support an important association between rs1800805 and rs6136 minor allele of SELP and increased risk of CVD, and additional interaction between rs1800805 and smoking.