Comparison of Solid Core HPLC Column Performance: Effect of Particle Diameter

In this technical note, the chromatographic performance of solid core 4 μm and 2.6 μm particle-packed columns is compared. Parameters compared are column pressure, efficiency, and impedance. pressure. There are other operating parameters that will have an impact on the overall system pressure. Some of these are the inner diameter and length of the connecting tubing in the LC system, the detector set-up parameters, such as flow cell volume in UV, or the inner diameter and length of the capillary components in ESI or APCI sources in LC/MS.