Study on the expression of 1 L-17 , TIM-1 and TIM-3 and the mechanism of insulin resistance in the periodontal tissues of rats with periodontitis and obesity

To analyze the expression change and mechanism of insulin resistance of IL-17, TIM-1 and TIM-3 in the double rat model of obesity with periodontitis. According to the sample size of each group, the animal models of normal group (A group), periodontitis (B group), obesity (C group), obesity with periodontitis (D group) were established respectively. The periodontal tissues and pancreatic tissues were observed by HE staining; immunohistochemical (IHC) staining was used to determine the number of 1L-17 positive cells and IRS-2 (insulin receptor substrate protein -2) and Caspase-3 positive cells; immunofluorescence double staining method was used to detect the expression of tryptasepositive MCs in TIM-1 and TIM-3. The expression level of IL-17 in the periodontal tissues of rats in D group was significantly higher than that of B group rats (P<0.05); and the level of IL-17 in the two groups showed a trend of increasing and then decreasing with the passage of time. There are both tryptase+MCs expression in TIM-1 and TIM-3 in gingival tissue with periodontitis, in D group, the double positive expression of tryptase-TIM-1 and tryptase-TIM-3 of mast cells in gingival tissue of rats was significantly higher than that of other groups (P<0.01). The expression of Caspsase-3 in pancreatic islets of rats in group C was (0.216±0.015), compared with the D group (0.320+0.022), the expression level was significantly lower, the difference was statistically significant; however, the expression level of IRS-2 in the pancreatic islets of rats in group C was (0.299+0.010), compared with D group, the expression level (2.210+0.011) decreased significantly (P<0.05). IL-17 can promote the development of periodontitis to some extent, and may be involved in the destruction and recovery of periodontal tissue; TIM-1 and TIM-3 play an important role in the pathogenesis and development of chronic periodontitis; and periodontitis leads to decrease of islet beta cells and decrease of function.