Comparison Studies Of Mineralized And Non-Mineralized Sf/Cs Hybrid Bone Scaffolds Co-Cultured With The Osteoblast Cell Line Mc3t3-E1 In Vitro

This work aimed to investigate the cytocompatibility and osteoinductivity of mineralized and non-mineralized silk fibroin/Chitosan (SF/CS) composite scaffolds co-cultured with the osteoblast cell line MC3T3-E1 in vitro. The mineralized (SF/CS/HA) and non-mineralized (SF/CS) scaffolds were fabricated utilizing freeze-drying method and biomimetic mineralization technique. The macrostructure and morphology as well as mechanical strength of two scaffolds were characterized. The MC3T3-E1 were cultured, expanded and seeded on SF/CS/HA and SF/CS scaffolds. The MC3T3-E1/scaffold constructs were cultured for 10 days and the adhesion, proliferation and osteogenic differentiation of MC3T3-E1 were determined using early adhesion ratio, cell counting kit-8 (CCK-8), alkaline phophatase (ALP) activity and scanning electronic microscopy (SEM). The two scaffolds had high porosity with interconnected porous architecture, and the mean porosity of SF/CS/HA, SF/CS scaffolds was 75.5% +/- 5.9% and 86.1% +/- 6.3%, respectively. The SF/CS/HA scaffold exhibited improved compressive strength and compressive modulus compatible with mechanical property of the cancellous bone. Significant proliferation on the SF/CS scaffold were observed while SF/CS/HA scaffold effectively promoted early cell attachment and enhanced osteogenic differentiation of MC3T3-E1 after 10 days of co-culture. The SF/CS/HA and SF/CS scaffolds were both biocompatible and had almost no negative effects on the MC3T3-E1 in vitro. However, the SF/CS/HA scaffold had better potential to be applied in bone tissue engineering for its good mechanical property and osteogenic differentiation.