PITC-2 Loaded Solid Lipid Nanoparticle: Design, Preparation, Characterization and Therapeutic Comparison with Free Phytochemical PITC-2 Isolated from Tissue Cultured Plant Pluchea Indica

Aim: The objective of the study is to design, preparation and characterization of PITC-2 loaded Solid Lipid Nanoparticle for i.v. administration is performed. Methods: Phytochemical PITC-2 is isolated from methanolic root extract of medicinal plant Pluchea indica which has a potent cytotoxic effect. PITC-2 loaded solid lipid nanoparticles were prepared by emulsion evaporation method. Formulation characterization was done using different technique and then pharmacological estimation of prepared SLNs was performed by comparing effects of PITC-2 SLNs with free phytochemical PITC-2 on ehrlich ascites carcinoma (EAC) cell in mice. Results: PITC-2 SLNs formed are smooth spherical particles observed in cryo-FESEM with less than 200nm in size. 52% encapsulation efficacy is found with a stable zeta potential of -35nV. DSC and PXRD studies indicate complete encapsulation of drug within the nanoparticle matrix in amorphous form. This formulation shows a sustained and prolonged drug release. Treatment with PITC-2 SLNs decreases tumor volume and increases lifespan of cancer bearing mice in comparison to phytochemical PITC-2. The histopathological examination also indicates that PITC-2 SLNs have promising apoptotic activity on tumor cells. Conclusion: The developed PITC-2 loaded SLNs can be used as drug carrier for sustained and prolonged drug release with improve therapeutic activity and bioavailability.