MiR-218-5 p Inhibited the Stemness Maintaining and Invasion of A 2 B 5 + / CD 133 – Subgroup Human Glioma Stem Cells

The article was aimed at detecting the expression of miR-218-5p in human gliomas, and exploring its influence in stemness maintaining and invasion of A2B5/CD133 human glioma stem cells (SHG139s). qRT-PCR was used to detect miR-218-5p expression in noncancerous brain tissue, human glioma tissue, human glioma cell lines and human glioma stem cell lines. Lentivirus vectors encoding miR-218-5p and antimiR-218-5p were constructed and stably transfected into A2B5/CD133 SHG-139s cells. Neurosphere formation assay, transwell assay, immunofluorescence and qRT-PCR analysis were used to explore the role of miR-2185p in SHG-139s. qRT-PCR analysis showed that miR-218-5p expression was lower in human glioma tissues and 网络出版时间:2015-03-30 15:56 网络出版地址:http://www.cnki.net/kcms/detail/31.2035.Q.20150330.1556.008.html