Reasearch on creating new germplasm of high oil content in B . napus

Microspore culture of four (F1) hybrid lines of B.napus was made and 1003 DH plantlets were obtained. By selection of 2 successive years for oil content, 16 new accessions were obtained whose oil content varied from 45% to 50.5% with adequate traits, days to flowering and maturing. This result suggested that microspore culture or doubled-haploid breeding would play an important role in creating new germplasm of B.napus, especially in polygenic traits such as oil content.