Expression of TGF-β_1 and TGF-β RII in retino-blastoma

Objective To investigate the expression of TGF-β1 and TGF-β RⅡ in retinoblastoma(Rb),and their relationships in clinical stage and prognosis.Methods Immunohistochemical technique was used to detect the expression of TGF-β 1 and TGF-β RⅡ in 37 cases of paraffin enbeded Rb samples. The quantitative analysis of the expression of TGF-β 1 was assessed by HPIAS-1000 Color Pathologic Analysis System.Results In all the 37 cases of Rb specimens,TGF-β 1 was expressed positively in tumor cells; The level of the expression of TGF-β 1 was significantly higher in tumor than in normal retina (P0.05)and there is significant difference between the optic nerve invasion group and the non-optic nerve invasion group (P0.05)and TGF-β 1 was higher in extra-eye stage than in glaucoma stage or intra-eye stage. TGF-β RⅡ was found negative in all 37 cases.Conclusion The increased expression of TGF-β 1 was significantly related to clinical stage and optic nerve invasion; the over expression of TGF-β 1 may indicate poor prognosis;the loss of response to the inhibitive effect of TGF-β 1 may contribute to the formation of Rb.