Biofilm formation of Staphylococcus, Streptococcus, Pasteurella and Neisseria strains.

The "in vitro" biofilm formation capacity of planktonic bacterial strains with zoonotic potential was tested on Staphylococcus, Streptococcus, Neisseria and Pasteurella strains from supragingival sites from dogs with dental disease, different breeds, age, sex, weight and diet. There were isolated and identified 75 strains, 24 belonging to the genus Pasteurella, ten to Staphylococcus genus, 28 to Steptococcus genus and 13 to Neisseria genus. After isolation and identification, these strains were tested for the in vitro biofilm formation capacity by microtitre plate assay. Results showed that all Staphylococcus, Streptococcus, Pasteurella and Neisseria species had the capacity to form biofilm in vitro, with higher or lower density. It was seen that there are interspecific and intraspecific differences between the ability of biofilm formation of these bacteria. The large frequency of the four bacterial genera from the oral cavity of all studied dogs emphasize the importance of knowing the frequency of isolation from the oral cavity and the presence of these microorganisms in association with dog bite wounds in humans.