The 10 μ L Dispensing Tool Ensures Highly Accurate and Precise Sub-microliter Volume Pipetting on epMotion ® 5073 l

This Application Note shows the possibility to automate quantitative Polymerase Chain Reaction (qPCR) setup involving small reaction volumes by using the epMotion® 10 μL dispensing tool. For PCR setup, a sample volume of 0.2 μL was used in a total reaction volume of 5 μL. The qPCR performances were evaluated by two sensitive qPCR assays. It was demonstrated that a low volume qPCR setup can be successfully automated on the epMotion 5073l. Excellent accuracy and reproducibility were obtained for both assays. The use of the epMotion workstation is an excellent solution to increase the consistency and efficiency of small volume dispensing by removing the human and day-today variability. In addition, researchers will save time and money by eliminating repetitive work and by reducing the use of costly reagents, respectively.