M A LDI-TOF MSI and electrochemical detection of metallothionein in chicken liver after cadmium exposure

MTs serve as cellular regulators by coordination of essential metals (Zn, Cu) and reducing the toxicity of heavy metals (Cd, Pb) [3]. MTs can be detected electrochemically in Brdicka solution. Here, three characteristic signals are measured for construction of electrochemical fingerprint of MT: RS2Co (about -1.3 V), Cat1 (-1.4 V) and Cat2 (-1.5 V). The obtained voltammograms are then analyzed by mathematical model [4,5]. Cadmium (Cd), a non-essential heavy metal and a selective toxicant, is accumulated mainly in the kidneys where it causes cellular injury [6]. Cd ions stimulate expression of MTs and toxicity occurs when the concentration of Cd ions exceeds the buffering capacity of intracellular MTs [7]. Cd naturally occurs in the environment as a pollutant from agriculture and industry [8-10]. Cadmium is a known teratogen M A LDI-TOF MSI and electrochemical detection of metallothionein in chicken liver after cadmium exposure Roman Guran1,2*, Iva Blazkova1,2, Renata Kensova1,2, Lukas Richtera1,2, Lucie Blazkova1,2, Ondrej Zitka1,2, Rene Kizek1,2 and Vojtech Adam1,2