The Influences of Chemotherapeutics on the Cell Cycle in SKN-SH Neuroblastoma Cells and Clinic Significance

This study was aimed to confirm the drug influence on cell cycle of SK-N-SH cells by flow cytometry analysis. The SK-N-SH cells were divided into two groups including Treatment group and control group. All of them were given chemotherapy drugs of ADM, CTX, VP16 and VCR, and then detected the influences of chemotherapy drugs on the cell cycle of SK-N-SH cells by flow cytometry instrument and. ADM: The percentage of G0/G1 phase of experimental group is (51.41±0.13)%, the percentage of G0/G1 phase of control group is (16.59±0.52)%. CTX:The percentage of G0/G1 phase of experimental group is (35.19±0.69)%, the percentage of G0/G1 phase of control group is (9.03±0.21)%. VP16:The percentage of G0/G1 phase of experimental group is (50.25±2.06)%, the percentage of G0/G1 phase of control group is (19.33±1.12)%. DDP:The percentage of G0/G1 phase of experimental group is (25.24±0.37)%, the percentage of G0/G1 phase of control group is (13.51±0.39)%. After treated with ADM, CTX, VP16 and DDP, the cell number of G0/G1 phase is more than control group in SK-N-SH cells, this showed significant difference (P<0.01). VCR:The percentage of G2/M phase of experimental group is (45.76±0.39)%, the percentage of G2/M phase of control group is (10.95±0.36)%. The cell number of G2/M phase is more than control group in SK-N-SH cells, this showed significant difference (P<0.01). ADM, CTX, VP16 and DDP can significantly arrest cell in G0/G1 phase, while VCR can significantly arrest cell in G2/M phase.