Circulating miR-665 and miR-30 c-1-3 p , the candidate markers of congestive heart failure with qi-deficiency-blood-stasis syndrome

According to the Chinese medicine (CM) theory, qi-deficiency-blood-stasis syndrome might be one of major patterns of congestive heart failure (CHF), but the underlying mechanism is yet not clear. Here, we aim to investigate the role of microRNAs (miRNAs) in the regulation of CHF in the presence of qi-deficiency-blood-stasis syndrome. We tested miRNAs expression microarray on the serum samples from CHF patients (three patients with qi-deficiencyblood-stasis syndrome, and three without), the significant different expressed miRNAs (DE-miRNAs) were screened and further quantified by RT-PCR in tissue samples. Target genes of these DE-miRNAs were analyzed using the software and miRNAs database, which could predict the relations between these DE-miRNAs and CHF mediated by qi-deficiency-blood-stasis syndrome. A total of 9 DE-miRNAs were confirmed, among them, miR-665 and miR-30c-13p draw specific attention. Because pathway analysis revealed that both miR-665 and miR-30c-1-3p target on the zinc finger protein 460 and 880, which translocated into nuclear and activated GATA4 transcription factor, the later was important for cardiac development survival and hypertrophy, therefore the upregulation of DE-miRNAs might be a biomarker to predict the progression of CHF. The occurrence and development of CHF may result from certain DE-miRNAs and their target genes participating in several signal pathways. MiR-665 and miR-30c-1-3p may be the candidate markers for the risk of congestive heart failure in Qi-deficiency-blood-stasis Syndrome.