Osteogenic differentiation of Wharton’s Jelly-derived mesenchymal stem cells

Induced osteogenesis of mesenchymal stem cells (MSCs) may provide an important tool for bone injuries treatment. Human umbilical cord is routinely discarded as clinical waste and may be used as noncontroversial MSCs sources. It still remains to be verified which source of MSCs is the most suitable for bone regeneration. The aim of this research was to investigate the osteogenic potential of MSCs derived from Wharton’s jelly of the human umbilical cord. Osteogenic differentiation of MSCs was detected and quantified by Alizarin Red S (ARS) staining for calcium deposition.