A Study on the Antibacterial Activity Of Zno Nanoparticles Prepared By Combustion Method against E Coli

Crystalline Zinc Oxide (ZnO) nanoparticles were synthesized by low temperature solution combustion method using Oxalyl dihydrazide (ODH) as fuel, at much lower temperature (300 o C). X-ray diffraction (XRD) confirmed the formation of wurtzite-structured pure ZnO No peaks from any else phases of ZnO and no impurity peaks were observed, indicating the high purity of the obtained hexagonal ZnO nanocrystals. The antibacterial activity of the formed nano ZnO were investigated against the pathogenic bacteria namely against E-coli. The bacteriological test is performed in Luria-Bertani and Nutrient agar media on solid agar plates and liquid broth system using different concentration of ZnO by standard microbial method. We have used both colony counting method and disk diffusion method. In both the methods ZnO nanoparticles with 100microg/L showed best antibacterial activity, and further studies on destruction of bacterial genomic DNA was done using PCR and gel electrophoresis revealed the DNA fragment bands, this activity might be due to surface charge interactions between the particles and cells. Free radical scavenging properties of the particles might have helped in cell wall disruption, and drastic antimicrobial action.