MiRNA-335-5 p negatively regulates granulosa cell proliferation via 1 SGK 3 in PCOS 2 3

MiRNA-335-5p negatively regulates granulosa cell proliferation via 1 SGK3 in PCOS 2 3 Lihua Yao 1, 2 , Mingyang Li 1, 2 , Jingwen Hu 1, 2 , Wangsheng Wang 1, 2 , Minzhi Gao 1, 2* 4 5 1. Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong 6 University, Shanghai, China. 7 2. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, 8 China 9 10 11 *Address all correspondence and requests for reprints to: Minzhi Gao, Ph. D. 12 Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong 13 University, Shanghai, China 14 Email: 328gao@ sina.com 15 16 17 Short title: MiRNA-335-5p and polycystic ovary syndrome 18 19 20 21 22 Page 1 of 30