Efficacy Of Phytopharmaceuticals From The Amazonian Plantlibidibia Ferreafor Wound Healing In Dogs

We comparatively evaluate two distinct formulations containing 5% of Juca (Libidibia ferrea) for wound healing in dogs. An excision model study was performed in 11 dogs with three dermal wounds in each animal, which were treated with: (1) topical phytopharmaceutical based on Carbopol (PyC) containing 5% Juca ethanolic extract; (2) topical phytopharmaceutical based onAstrocaryum murumurubutter (PyM) containing 5% Juca ethanolic extract; and (3) commercial ointment (control). Wound treatment was carried out on alternated days starting at day (D) one until D21. Macroscopic (all time-points) and histological (D0 and D21) analyses were performed. The antimicrobial activity of Juca was evaluated through Minimal Inhibitory Concentration (MIC). Phytochemical analysis of Juca revealed 3.1% phenolic compound content expressed in rutin and the presence of hydrolyzable tannins and flavonoids. The mean wound retraction was 33.7 +/- 5.5, 34.0 +/- 4.7, and 28.4 +/- 4.9 % for PyC, PyM, and control groups, respectively, with higher wound retraction for both herbal-treated groups compared to the control (P< 0.05). Alcoholic extract of Juca had antimicrobial activity against the microorganismsStaphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, andCandida kruseiat different degrees, with MIC ranging from 250 to 16.625 mu g/ml. Microscopic evaluation showed that the phytotherapic formulations contributed to better dermal wound healing through wound fibroplasia. The alcoholic extract of Juca pods has great potential for wound healing in dogs and can be used in the development of commercially viable phytotherapic formulations.