A High-Throughput System for Cyclic Stretching of Precision-Cut Lung Slices During Acute Cigarette Smoke Extract Exposure

Rationale Precision-cut lung slices (PCLSs) are a valuable tool in studying tissue responses to an acute exposure; however, cyclic stretching may be necessary to recapitulate physiologic, tidal breathing conditions. Objectives To develop a multi-well stretcher and characterize the PCLS response following acute exposure to cigarette smoke extract (CSE). Methods A 12-well stretching device was designed, built, and calibrated. PCLS were obtained from male Sprague-Dawley rats (N= 10) and assigned to one of three groups: 0% (unstretched), 5% peak-to-peak amplitude (low-stretch), and 5% peak-to-peak amplitude superimposed on 10% static stretch (high-stretch). Lung slices were cyclically stretched for 12 h with or without CSE in the media. Levels of Interleukin-1 beta (IL-1 beta), matrix metalloproteinase (MMP)-1 and its tissue inhibitor (TIMP1), and membrane type-MMP (MT1-MMP) were assessed via western blot from tissue homogenate. Results The stretcher system produced nearly identical normal Lagrangian strains (E(xx)andE(yy),p> 0.999) with negligible shear strain (E-xy< 0.0005) and low intra-well variability 0.127 +/- 0.073%. CSE dose response curve was well characterized by a four-parameter logistic model (R-2= 0.893), yielding an IC(50)value of 0.018 cig/mL. Cyclic stretching for 12 h did not decrease PCLS viability. Two-way ANOVA detected a significant interaction between CSE and stretch pattern for IL-1 beta (p= 0.017), MMP-1, TIMP1, and MT1-MMP (p< 0.001). Conclusion This platform is capable of high-throughput testing of an acute exposure under tightly-regulated, cyclic stretching conditions. We conclude that the acute mechano-inflammatory response to CSE exhibits complex, stretch-dependence in the PCLS.