Heterologous Expression And Characterization Of Flavinadenine Dinucleotide Synthetase From Candida Famata For Flavin Adenine Dinucleotide Production

Background: Flavin adenine dinucleotide (FAD) is a redox-active coenzyme that regulates several important enzymatic reactions during metabolism. FAD is used in the medicinal and food industries and FAD supplements have been used to treat some inheritable diseases. FAD can be biosynthesized from flavin mononucleotide (FMN) and adenosine triphosphate (ATP), catalyzed by FAD synthetase (FADS).Objective: The aim of this study was to heterologously express the gene encoding FADS from the flavinogenic yeast Candida famata (FADS(Cf)) for biosynthesis of FAD.Methods: The sequence encoding FADS(Cf) was retrieved and heterologously expressed in Escherichia coli. The structure and enzymatic properties of recombinant FADS(Cf) were characterized.Results: FADS(Cf) (279 amino acids) was successfully expressed in E. coli BL21 (DE3), with a theoretical molecular weight of 32299.79 Da and an isoelectric point of 6.09. Secondary structural analysis showed that the number of alpha-helices was 2-fold higher than the number of beta-sheets, indicating that the protein was highly hydrophilic. Under fixed ATP concentration, FADS(Cf) had a K-m of 0.04737 +/- 0.03158 mM and a V-max of 3.271 +/- 0.79 mu M/min/mg. Under fixed FMN concentration, FADS(Cf) had a K-m of 0.1214 +/- 0.07464 mM and a V-max of 2.6695 +/- 0.3715 mu M/min/mg. Enzymatic reactions in vitro showed that expressed FADSCf could form 80 mM of FAD per mg of enzyme after 21 hours under the following conditions: 0.5 mM FMN, 5 mM ATP and 10 mM Mg2+.Conclusion: Under optimized conditions (0.5 mM FMN, 5 mM ATP and 10 mM Mg2+), the production of FAD reached 80 mM per mg of FADS(Cf) after a 21-hour reaction. Our results indicate that purified recombinant FADS(Cf) can be used for the biosynthesis of FAD.