The Molecular Mechanisms Of Probiotic Strains In Improving Ageing Bone And Muscle Ofd-Galactose-Induced Ageing Rats

Aim The aim of this study was to evaluate the molecular mechanisms ofLactobacillusstrains in improving ageing of the musculoskeletal system. Methods and Results The anti-ageing mechanism of three probiotics strainsLactobacillus fermentumDR9,Lactobacillus paracaseiOFS 0291 andL. helveticusOFS 1515 were evaluated on gastrocnemius muscle and tibia ofd-galactose-induced ageing rats. Upon senescence induction, aged rats demonstrated reduced antioxidative genes CAT and SOD expression in both bone and muscle compared to the young rats (P < 0 center dot 05). StrainL. fermentumDR9 demonstrated improved expression of SOD in bone and muscle compared to the aged rats (P < 0 center dot 05). In the evaluation of myogenesis-related genes,L. paracaseiOFS 0291 andL. fermentumDR9 increased the mRNA expression of IGF-1;L. helveticusOFS 1515 andL. fermentumDR9 reduced the expression of MyoD, in contrast to the aged controls (P < 0 center dot 05). Protective effects ofL. fermentumDR9 on ageing muscle were believed to be contributed by increased AMPK-alpha 2 expression. Among the osteoclastogenesis genes studied, TNF-alpha expression was highly elevated in tibia of aged rats, while all three probiotics strains ameliorated the expression.Lactobacillus fermentumDR9 also reduced the expression of IL-6 and TRAP in tibia when compared to the aged rats (P < 0 center dot 05). All probiotics treatment resulted in declined proinflammatory cytokines IL-1 beta in muscle and bone. Conclusions Lactobacillus fermentumDR9 appeared to be the strongest strain in modulation of musculoskeletal health during ageing. Significance and Impact of the Study The study demonstrated the protective effects of the bacteria on muscle and bone through antioxidative and anti-inflammatory actions. Therefore,L. fermentumDR9 may serve as a promising targeted anti-ageing therapy.