苜蓿链霉菌内切β-N-乙酰氨基葡萄糖苷酶的克隆、表达及酶学性质
Sheng wu gong cheng xue bao = Chinese journal of biotechnology(2020)
Abstract
内切β-N-乙酰氨基葡萄糖苷酶广泛应用于糖生物学研究和工业生产.本研究从苜蓿链霉菌Streptomyces alfalfae ACCC 40021中克隆并原核表达了一个新的内切β-N-乙酰氨基葡萄糖苷酶,该酶最适反应温度为35℃,最适pH为6.0,具有良好的pH稳定性、温度稳定性和高比活(1×106 U/mg)的特性,可催化不同蛋白底物去糖基化,具有作为工具酶和生物催化剂的潜力.
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Key words
Streptomyces alfalfae,deglycosylation,endo-β-N-acetylglucosaminidase
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