Comparison Of The Diagnostic Performance Of Microscopic Examination, Copro-Elisa, And Copro-Pcr In The Diagnosis Ofcapillaria Philippinensis Infections

Intestinal capillariasis is a parasitic zoonosis caused by the tiny nematode parasiteCapillaria philippinensis. It is a major health problem that may lead to death if not diagnosed and treated appropriately. The difficulties in the diagnosis ofC.philippinensishighlight the importance of developing accurate, sensitive, and specific methods for early diagnosis. This study aimed to detect the presence ofC.philippinensisinfection among 42 clinically suspected patients with certain criteria that are highly suggestive of capillariasis and to compare the diagnostic yield of microscopy, copro-ELISA, and PCR for the detection of copro-DNA. Sociodemographic characteristics and clinical data were also described for the infected group. Out of 42 patients, 10 were microscopically positive, 40 samples were positive by copro-ELISA, nested PCR detected 35 positive cases, with total detection rates of 23.8%, 95.2%, and 83.3% using direct microscopic examination, copro-ELISA, and PCR, respectively. The majority of positive cases were females, middle-aged people, and people from rural areas. The real number of cases infected withC.philippinensismay far exceed those estimated using microscopy. The diagnosis by copro-ELISA for the detection ofC.philippinensiscoproantigen and by nested PCR to identify parasite DNA revealed a higher number of positive cases. Using ELISA for the detection of coproantigen is a sensitive test that identifies the infection, yet it is not specific. Copro-DNA offers a satisfactory sensitive and specific method for the detection of infection in clinically suspected patients. The most susceptible individuals toC.philippinensisinfection are females, middle-aged people, and people of low social standards. Intestinal capillariasis needs to be considered in patients who present with symptoms of chronic diarrhea and hypoalbuminemia because if these cases are left undiagnosed and untreated, they may suffer from lethal complications.