Comparison of five different laboratory techniques for the rabies diagnosis in clinically suspected cattle in Brazil.

N H F Centoamore, M E R Chierato, V B V Silveira,K M Asano,K Iamamoto,W O Fahl,K C Scheffer,S M Achkar,L P Mesquita,P C Maiorka,E Mori

Journal of Virological Methods（2020）

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摘要

•If dFAT results are negative or inconclusive, rabies diagnosis may require auxiliary tools.•RABV non-homogenous distribution in CNS may interfere in the diagnosis.•Molecular techniques can detect RABV in low viral load samples.

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关键词

Rabies,Laboratory diagnosis,Bovine,Direct-fluorescent antibody test (dFAT),Immunohistochemistry (IHC),Reverse transcriptase PCR (RT-PCR),Real-time reverse transcriptase PCR (RT-qPCR)

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