PO-064 Tubulin-binding anti-cancer polysulfides induce cell death via mitotic arrest and autophagic interference in colon cancer

Introduction Colon cancer is a major cause of morbidity and mortality worldwide. Epidemiological studies revealed an inverse correlation between colon cancer risk and a garlic-rich diet. Natural organosulfur compounds confer protective effects against a wide range of cancer, including colon. Here, we studied the anti-colon cancer activity of garlic-derived diallyl/dibenzyl tetrasulfur (DATTS/DBTTS) derivatives.Material and methods We validated the ability of DATTS/DBTTS to bind tubulin by MALDI-TOF mass spectrometry and its disruptive effect on the microtubule network by immunofluorescence. We selected cell lines with a defined genetic background including HT-29 (BRAF-mutation), SW480 and metastatic SW620 (both KRAS-mutation). Cell cycle analysis, Hoechst staining and western blots allowed to assess cell death. To further validate the anti-cancer activity of DBTTS, colony and spheroid formation assays …