New Determinants Of Aminoglycoside Resistance And Their Association With The Class 1 Integron Gene Cassettes Intrueperella Pyogenes

Trueperella pyogenesis an important opportunistic animal pathogen. Different antimicrobials, including aminoglycosides, are used to treatT. pyogenesinfections. The aim of the present study was to evaluate aminoglycoside susceptibility and to detect aminoglycoside resistance determinants in 86T. pyogenesisolates of different origin. Minimum inhibitory concentration of gentamicin, streptomycin, and kanamycin was determined using a standard broth microdilution method. Genetic elements associated with aminoglycoside resistance were investigated by PCR and DNA sequencing. All studied isolates were susceptible to gentamicin, but 32.6% and 11.6% of them were classified as resistant to streptomycin and kanamycin, respectively. A total of 30 (34.9%) isolates contained class 1 integrons. Class 1 integron gene cassettes carrying aminoglycoside resistance genes,aadA11andaadA9, were found in seven and two isolates, respectively. Additionally, theaadA9gene found in six isolates was not associated with mobile genetic elements. Moreover, other, not carried by gene cassettes, aminoglycoside resistance genes,strA-strBandaph(3')-IIIa, were also detected. Most importantly, this is the first description of all reported genes inT. pyogenes. Nevertheless, the relevance of the resistance phenotype to genotype was not perfectly matched in 14 isolates. Therefore, further investigations are needed to fully explain aminoglycoside resistance mechanisms inT. pyogenes.