A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes

Irena Hilje-Rodríguez,Federico J. Albertazzi, German Rivera-Coto,Ramón Molina-Bravo

Biotechnology Reports（2020）

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摘要

•Quantitative polymerase chain reactions specifically detect Trichoderma atroviride.•The method uses a probe based on the tef-1α for the detection.•The method simultaneously detects T. atroviride and Botrytis cinerea in blackberries.

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关键词

Biological control,Molecular quantification,Hydrolysis probe,qPCR,Rubus adenotrichos

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