Poor Stimulation Of Bovine Dendritic Cells Bymycobacterium Bovisculture Supernatant And Surface Extract Is Associated With Decreased Activation Of Erk And Nf-Kappa B And Higher Expression Of Socs1 And 3

The cell envelope of pathogenic mycobacteria interfaces with the host. As such, the interaction of bacterial products localized at or released from the cell surface with the host's immune system can determine the fate of the bacterium in its host. In this study, the effects of three different types ofMycobacterium boviscell envelope fractions-purified protein derivative, total cell wall lipids and culture supernatant and surface extract-on bovine dendritic cells were assessed. We found that the culture supernatant and surface extract fraction induced little to no production of the pro-inflammatory cytokines TNF-alpha and IL-12 in bovine dendritic cells. Moreover, this muted response was associated with poor activation of ERK and NF-kappa B, both of which are critical for the pro-inflammatory response. Furthermore, culture supernatant and surface extract treatment increased the expression of suppressor of cytokine signaling 1 and 3, both of which are negative regulators of pro-inflammatory signaling, in bovine dendritic cells. These observations taken together suggest theM. bovisculture supernatant and surface extract fraction contain immunomodulatory molecules that may aid inM. bovispathogenesis.