The polymorphism of rs11614913 T/T in pri- miR-196a-2 alters the miRNA expression and associates with recurrent spontaneous abortion in a Han-Chinese population.

Rs11614913 in pri- is involved in the occurrence of many diseases, especially in cancers. However, it remains unknown whether is associated with human recurrent spontaneous abortion (RSA) in Chinese Han population. Our study found that rs11614913 T/T in pri- was associated with the increase risk of human unexplained RSA (URSA) in recessive mode in Chinese Han population. The T allele of rs11614913 increased the production of mature . Rs11614913 T/T inhibited HTR-8/SVneo cells proliferation and migration and promoted cells apoptosis. Further investigation discovered that dihydrofolate reductase () was the target of and inversely regulated by . Dual-luciferase assay indicated that T allele in rs11614913 could more effectively suppress expression than C allele. In addition, C to T substitution in rs11614913 attenuated the sensibility of cells to mifepristone. Collectively, our data suggest that rs11614913 T/T in pri- may be conductive to the genetic predisposition to RSA by disrupting the production of mature and reinforcing the expression of .