Estimation of optimum injection volume during preparative chiral separation.
Journal of Chromatography A(2020)
摘要
•For maximizing throughput it is important to optimize preparative injection volume.•In industry optimum volume is detected iteratively through multiple experiments.•This report provides a formula to estimate optimum injection volume.•Formula applicable to both HPLC and SFC, peak following Langmuir isotherm.•Example provided with this report is of a preparative SFC separation.
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关键词
Preparative,SFC,Supercritical Fluid Chromatography,Injection volume,Chiral separation,Enantiomers,Mathematical model
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