A new application of inorganic sorbent for biomolecules: IMAC practice of Fe 3+ -nano flowers for DNA separation.

Selection of purification method and type of adsorbent has high significance for separation of a biomolecule like deoxyribonucleic acid (DNA). Nanoflowers are a newly improved class of adsorbent. Due to showing very structural similarity to plant flowers, they are named as nanoflowers. Herein, after synthesize of copper phosphate three hydrate nanoflowers [(Cu3(PO4)2.3H2O), CP-NFs], Fe3+ ions were attached to their surfaces. Obtained Fe3+-CP-NFs, before investigation of some adsorption parameters for DNA, they were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Some attained data from the results of adsorption experiments as follows: While maximum DNA adsorption on Fe3+-CP-NFs was found as an excellent value of 845.8 mg/g, nanoflowers without Fe3+ ions adsorbed DNA as only 25.3 mg/g. Optimum media conditions for DNA adsorption were observed at pH 7 and 25 °C with an initial concentration of 1.5 mg/mL DNA. Langmuir and Freundlich adsorption equations were applied to determine which adsorption model was appropriate, and it was seen that Langmuir model was fit with a R2 of 0.9885.