Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR.

•Development and complete validation of a one-step RT-qPCR method for the detection and quantification of PEDV.•Broad range detection of S-INDEL and S-non-INDEL strains.•Optimization of Primer concentrations reduce primer dimer formation.•Addition of a proteinase K treatment allow good reproducibility.