An integrated pipeline for high-throughput screening and profiling of spheroids using simple live image analysis of frame to frame variations

Haneen Alsehli,Fuad Mosis,Christopher Thomson,Eva Hamrud,Erika Wiseman,Eileen Gentleman,Davide Danovi

Methods（2021）

引用 14|浏览13

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摘要

•Human induced pluripotent stem cells in self-renewing or differentiating conditions.•Simple live phase images analysed using CellProfiler and frame to frame subtraction.•Convolutional Neuronal Network predicts culture condition from spheroid morphology.

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关键词

High content imaging,High throughput imaging,Cell phenotyping,3D,Spheroids,Stem cells

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